Anna Boguszewska-Chachulska, PhD    

EMPLOYMENT:

• 2013 CEO, Genomed SA (joint-stock company)
• 2009-2013 COO, Member of the board of Directors, Genomed Ltd, since 2011 - Genomed SA (joint-stock company)
• 2007-2009 Genomed Ltd. Co. - co-founder and member of the Advisory Board
• 2009-2010 Professor at the Institute of Biotechnology, Department of Chemistry, Warsaw University of Technology
• 2008-Associate Professor at the Institute of Biochemistry and Biophysics
• 1998-2008 Assistant Professor at the Institute of Biochemistry and Biophysics
• 1993-1998 Senior research assistant, Institute of Biochemistry and Biophysics
• 1990-1993 Research assistant

EDUCATION:

• Habilitation in Biology Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, October 2008
• Ph.D. in Biochemistry: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, January 1998
• Studies: Biology Faculty, University of Warsaw, 1984-1990
• Specialisation: Molecular Biology
• M.Sc.: Department of Genetics, University of Warsaw, 1990
• B.Sc.(Equivalent): Biology Faculty, University of Warsaw, 1990

RESEARCH TRAINING AND EXPERIENCE:

• August 1998 – June 2000 – post-doctoral training in the laboratory of Dr. Linda E. Hyman at Tulane University in New Orleans in U.S. – looking for the roles of Elongin C in yeast
• 1996-1998 - leading a project on plant transformation towards potato virus Y resistance financed by a grant of Polish Committee of Scientific Research
• Laboratoire de Biologie Cellulaire, INRA, Versailles, France - CNRS stipend, October-December 1992, December 1993; October 1995, 1996, 1997
• EEC grant - April-June 1993, French Government stipend - July-August 1995

TEACHING EXPERIENCE:

• 2019-2024 Co-tutor of two PhD theses
• 2009-2010 Tutor of two M.Sc. and three B.Sc. students
• 2004-2009 Tutor of two PhD theses and co-tutor of one PhD thesis

AWARDS:

• December 2017 the 2nd place in the first "10" of the most enterprising women in the category of companies below PLN 50 million turnover per year.
• May 2011 Genomed S.A. received the Master of Business award in the category of Innovation
• December 04 2006 Habilitation stipend for Women in Science 2006 awarded by l’Oréal Polska with a support from Polish UNESCO Commitee
• March 23 2006 Award of IBB PAS for the review published in Prog. Nucleic Acid Res. Mol. Biol. 80, 135-68. “Virus-Like Particles: Models for Assembly Studies and Foreign Epitope Carriers.”
• November 18 1999 Award of Polish Academy of Sciences for co-authoring of a series of works dedicated to “RNA genomes of crop pathogens – structures, functions, control and spread”
• June 30 1998 Polish Scientific Award of Plant Genetics - First prize for “Creative contribution to the plant molecular virology”

LIST OF PUBLICATIONS:

• Wąsowska A, Sendecki A, Boguszewska-Chachulska A, Teper S. Polygenic Risk Score and Rare Variant Burden Identified by Targeted Sequencing in a Group of Patients with Pigment Epithelial Detachment in Age-Related Macular Degeneration. Genes 2023;14:1707. doi.org/10.3390/genes14091707
• Krytkowska E, Ulańczyk Z, Grabowicz A, Safranow K, Kawa MP, Pałucha A, Wąsowska A, Matczyńska E, Boguszewska-Chachulska A, Machalińska A. Influence of Clinical and Genetic Factors on the Progression of Age-Related Macular Degeneration: A 3-Year Follow-Up. J Clin Med. 2023;12(5):1963. doi: 10.3390/jcm12051963
• Wąsowska A, Teper S, Matczyńska E, Łyszkiewicz P, Sendecki A, Machalińska A, Wylęgała E, Boguszewska-Chachulska A. Polygenic Risk Score Impact on Susceptibility to Age-Related Macular Degeneration in Polish Patients.  J Clin Med. 2022;12(1):295. doi: 10.3390/jcm12010295
• Musialik J, Boguszewska-Chachulska A, Pojda-Wilczek D, Gorzkowska A, Szymańczak R, Kania M, Kujawa-Szewieczek A, Wojcieszyn M, Hartleb M, Więcek A. A Rare Mutation in The APOB Gene Associated with Neurological Manifestations in Familial Hypobetalipoproteinemia. Int J Mol Sci. 2020;21(4):1439. doi: 10.3390/ijms21041439
• Oliver KL, Franceschetti S, Milligan CJ, Muona M, Mandelstam SA, Canafoglia L, Boguszewska-Chachulska AM, Korczyn A, Bisulli F, Di Bonaventura C, Ragona F, Michelucci, Ben-Zeev B, Straussberg R, Panzica F, Massano J, Friedman D, Crespel A, Engelsen BA, Andermann F, Andermann E, Spodar, Lasek-Bal A, Riguzzi P, Pasini E, Tinuper P, Licchetta L, Gardella E, Lindenau M, Wulf A, Møller RS, Benninger F, Afawi Z, Rubboli G, Reid CA, Maljevic S, Lerche H, Lehesjoki AE, Petrou S, Berkovic SF. Myoclonus epilepsy and ataxia due to KCNC1 mutation: Analysis of 20 cases and K+ channel properties. Ann Neurol. 2017;81(5):677-689
• Stankiewicz-Drogoń A, Dörner B, Erker T, Boguszewska-Chachulska AM. Synthesis of new acridone derivatives, inhibitors of NS3 helicase, which efficiently and specifically inhibit subgenomic HCV replication. 2010 J Med Chem. 53, 3117-26
• Najda-Bernatowicz A, Krawczyk M, Stankiewicz-Drogoń A, Bretner M, Boguszewska-Chachulska AM. Studies on the anti-hepatitis C virus activity of newly synthesized tropolone derivatives: identification of NS3 helicase inhibitors that specifically inhibit subgenomic HCV replication. 2010 Bioorg Med Chem. 18, 5129-36
• Krawczyk M., Stankiewicz-Drogoń A., Haenni A.-L., and Boguszewska-Chachulska A. Fluorometric assay of helicase activity w: Helicases. Methods and Protocols, Series: Methods in Molecular Biology, Vol.587 Abdelhaleem, Mohamed M. (Ed.) 2010, XII,ISBN: 978-1-60327-354-1
• Krawczyk M., Wasowska-Lukawska M., Oszczapowicz I., Boguszewska-Chachulska A.M., Amidinoanthracyclines - a new group of potential anti-hepatitis C virus compounds. 2009. Biol. Chem. 390, 351-360
• Stankiewicz-Drogon A., Palchykovska L.G., Kostina V.G., Alexeeva, I.V., Shved A.V., and Boguszewska-Chachulska A.M. New acridone-4-carboxylic acid derivatives as potential inhibitors of Hepatitis C virus infection. 2008. Bioorg. Med. Chem. 16, 8846-8852
• Gozdek A., Poznański, J., Stankiewicz-Drogoń A. and Boguszewska-Chachulska A.M Circular dichroism analysis for multidomain proteins: studies of the irreversible unfolding of Hepatitis C virus helicase.” 2008. Acta Biochim. Pol. 55, 57-66
• Gozdek A., Zhukov I., Polkowska A., Poznanski J., Stankiewicz-Drogon A., Pawlowicz J.M., Zagórski-Ostoja W., Borowski P., and Boguszewska-Chachulska A.M. NS3 peptide, a novel potent Hepatitis C virus NS3 helicase inhibitor, its mechanism of action and antiviral activity in the replicon system. 2008. Antimicrob. Agents Chemother. 52, 393-401
• Boguszewska-Chachulska AM, Krawczyk M, Najda A, Kopanska K, Stankiewicz-Drogon A, Zagorski-Ostoja W, Bretner M. Searching for a new anti-HCV therapy: Synthesis and properties of tropolone derivatives. 2006. Biochem Biophys Res Commun. 341, 641-647
• Palucha A., Loniewska A., Satheshkumar S., Boguszewska-Chachulska A.M., Umashankar M., Milner M., Haenni A.-L. and Savithri H.S. Virus-Like Particles: Models for Assembly Studies and Foreign Epitope Carriers. 2005. Progress Nucl. Acid Res. Mol. Biol. 80, 135-168
• Boguszewska-Chachulska A.M and Haenni A.-L. RNA viruses redirect host factors to better amplify their genome. 2005. Adv. Virus Res. 65, 29-61
• Boguszewska-Chachulska A.M., Krawczyk M., Stankiewicz A., Gozdek A., Haenni A.-L., Strokovskaya L. Direct fluorometric measurement of hepatitis C virus helicase activity. 2004, FEBS Lett. 567, 253-258
• Hyman L.E., Kwon E., Ghosh S., McGee J., Chachulska A.M., Jackson T., Baricos W.H. Binding to elongin C inhibits degradation of interacting proteins in yeast. 2002. J. Biol. Chem. 277, 15586-15591
• Zagórski W., Chachulska A., Lipska-Dwużnik A., Flis B., Pałucha A. Molecular phylogeny as a tool for controlling Potato Virus Y spread. Use of agriculturally important genes in biotechnology. G. Hradzina (Ed.) IOS Press, 2000, 148-152
• Flis B., Chachulska A., Pałucha A. Performance of virus resistant transgenic potatoes. Use of agriculturally important genes in biotechnology. G. Hradzina (Ed.) IOS Press, 2000, 142-147 Doroszewska T., Chachulska A.M. Możliwości uzyskania odporności na choroby wirusowe tytoniu. 2000, Biotechnologia 4 (51), 128-141
• Jackson T., Kwon E., Chachulska A.M., Hyman L.E. “Novel roles of Elongin C in yeast.” 2000. Bioch. Biophys. Acta 1491, 161-176
• Chachulska A.M., Chrzanowska M., Robaglia C. and Zagórski W. “Tobacco veinal necrosis determinants are unlikely to be located within the 5’ and 3’ terminal sequences of the potato virus Y genome.” 1997, Arch. Virol., str. 67, 189-197
• Chachulska A.M., Fakhfakh H., Robaglia C., Granier F., Zagórski W. and Vilaine F. Synthesis of potyvirus full-length cDNA copies suitable for the analysis of genome polymorphism. 1997, J. Virol. Methods 67, 189-197
• Chachulska A.M., Chrzanowska M., Flis B., Krzymowska M., Lipska-Dwużnik A., Robaglia C. and Zagórski W. Potato and tobacco cultivars transformation towards potato virus Y resistance. 1997, Biotechnologia 4 (39), str. 38-48.
• Chrzanowska M., Doroszewska T., Chachulska A. Charakterystyka szczepów wirusa Y występujących w Polsce w uprawach tytoniu i ziemniaka. 1997, Progress in Plant Protection/ Postępy w Ochronie Roślin 37 (2), str. 327-329
• Marie-Jeanne V., Chachulska A.M., Fakhfakh H., Le Romancer M., Robaglia C. et Astier-Manifacier S. "Polymorphisme de sequence dans la region 5' non-codante et P1 de l'ARN de PVY." 1995 , Annales du Tabac, 27(2), str. 59-64
• Chachulska A.M. i Zagórski W. “Analiza sekwencyjna genomów izolatów wirusa Y ziemniaka” w “ Wybranych zagadnieniach biotechnologii roślin” pod redakcją K. Wypijewskiego. 1996. str. 71-83
• Marie-Jeanne Tordo V., Chachulska A.M., Fakhfakh H., Le Romancer M., Robaglia C. and Astier-Manifacier S. "Sequence polymorphism in the 5'NTR and in the P1 coding region of Potato Virus Y genomic RNA.", 1995, J.Gen.Virol., 76, str. 939-949
• Chachulska A.M."Rybozymy - katalityczne czasteczki RNA", 1992, Postepy Biochemii, 38, str.64-74

PATENTS OBTAINED:

• Pat.227811 „Diagnostic set and method for simultaneous detection of mutations within the NF1 gene for the group of patients with suspected Recklinghausen disease” filed on 30.06.2014
• PAT.222649 „Method and diagnostic kit for the detection of mutations in the I gene neurofibromin and in patients with suspected neurofibromatosis I type”, fied on 18.12.2012
  
• P-186760 (1997) New binary vector and method of its construction P-186761 (1997) Method of construction of plants resistant to potato virus Y (PVY)
  

PATENT APPLICATIONS FILLED:

• P 369883 „Tropolone analogs as inhibitors of NTPase/helicase of viruses belonging to the Flaviviridae family and as antiviral agents”, filed on 09.03.2004
• P 381377 "New acridone derivatives, method of preparation and their application " filed on 12.21.2006
• P 381714 „New medical applications of anthracycline antibiotics’ derivatives” filed on 02.07.2007 in the Polish Patent Office, and on 02.07.2008 in the European Patent Office, application No PCT/PL2008/000010