DNA Sequence Analysis
Services offered:
- Assembly of single readings into longer sequences. Creation of single contigs.
- Primer design and set-up of PCR and sequencing conditions.
- Restriction analysis of DNA fragments,creation of restriction maps.
- Search for open reading frames (ORFs) and ORF analysis; identification of protein motifs and cellular localisation signals.
- Search for homologous sequences and creation of phylogenetic trees
- Mutation analysis, comparison of the given sequence with the reference one.
Useful Links:
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Align two sequences using BLAST (bl2seq)
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Nucleotide blast
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Oligo Calc: Oligonucleotide Properties Calculator
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Reverse Complement
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Pick primers from a DNA sequence : Primer 3.0
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UCSC In-Silico PCR