We offer the synthesis of DNA and RNA oligos, fluorescent and other oligonucleotide modifications as well as purification using the HPLC method.

DNA oligonucleotides:

standard oligonucleotides

synthesis scale [umol] (µmol)	purification standard ¹		purification HPLC
synthesis scale [umol] (µmol)	price per base ² (netto PLN)	minimum yield OD ₍₂₆₀₎³	price per base (netto PLN)	minimum yield OD ₍₂₆₀₎
0.02⁴	1.1	3	3.45	1
0.04	1.3	5	3.65	2.5
0.20	2.5	16	5	8
1.00	9.9	80	12	25

¹ Standard desalting consists in ethanol precipitation. It guarantees a level of purification sufficient for standard applications (PCR reaction and sequencing)
² For oligonucleotides longer than 80 bp price is 110% higher. For oligonucleotides longer than 90 bp price is 120% higher. For oligonucleotides shorter than 15 bp price calculated as for 15-mer..
³ Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed.
⁴ 0.02 µmol scale only for oligos up to 33 bases.

antisense oligonucleotides (PTOs)
labelled oligonucleotides

RNA oligonucleotides:

deoxynucleotides

mi-N1005S dNTP Set 100 mM (in water, 4x 250 µl) - 390 PLN net price
mi-N1006M dNTP Mix 10 mM (in water, 250 µl) - 110 PLN net price

Delivery:

DNA and RNA oligos are delivered in a lyophylised form along with an information concerning the quantity in which a given lyophylisate should be resuspended to obtain 100 µM; concentration.
We advise 10 mM Tris pH 7.5. when resuspending DNA oligos.

Orders:

Please order online by filling out the form. If you encounter any problem while ordering, please do it by e-mail.

Order processing time:

standard oligonucleotides: 2-3 working days.
standard oligonucleotides with HPLC purification: do 5 working days.
labelled oligonucleotides: do 10 working days.
RNA oligonucleotides: please contact us

Delivery costs:

When your orders value is greater than 200 PLN Genomed covers the costs of delivery mail in Poland.
Orders for less than 200 PLN net - delivery cost in Poland - 30 PLN netto
Primers can also be shipped by registered letter free of charge.

Prices:

Prices are listed in the tables below. With the help of the oligonucleotides ordering form it is possible to calculate the cost of an oligonucleotide.

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standard oligonucleotides

Net prices (PLN), minimum yield and offered synthesis scale of standard oligonucleotides:

standard oligonucleotides
synthesis scale [umol] (µmol)	purification standard ¹		purification HPLC
synthesis scale [umol] (µmol)	price per base ² (netto PLN)	minimum yield OD ₍₂₆₀₎³	price per base (netto PLN)	minimum yield OD ₍₂₆₀₎
0.02⁴	1.1	3	3.45	1
0.04	1.3	5	3.65	2.5
0.20	2.5	16	5	8
1.00	9.9	80	12	25

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antisense oligonucleotides (PTOs)

Net prices (PLN), minimum yield and offered synthesis scale of phosphorothioates (PTOs, S-oligos).
Prices include HPLC- and cartridge-purification, if the oligo is a "Full-PTO"!
For mixed Phosphosphorothioate/Phosphorodiester-Oligos (i. e. "end capping") cartridge-purification will be charged at 65 PLN net price. We recommend this service

antisense oligonucleotides (PTOs)
synthesis scale [umol] (µmol)	purification HPLC
synthesis scale [umol] (µmol)	price per base (netto PLN)	minimum yield OD ₍₂₆₀₎¹
0.20	13	5
1.00	27	15
10.00	92	80

¹ Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed.
A higher scale of synthesis - please contact us

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labelled oligonucleotides

Genomed offers labelling of oligos at 5' and 3' ends as well as internal modifications. If the type of labelling you are interested in is not to be found on the price list, please enquire.

Synthesis of labelled oligonucleotides, offered scales, minmal amount and net prices:

fluorescent labels of 5' end¹
purification:	standard				HPLC
synthesis scale [umol] (µmol): minimum yield (OD₂₆₀):	0.02 3	0.04 5	0.20 12	1.00 50	0.02 1	0.04 2	0.20 5	1.00 12
6-FAM	88	95	147	278	98	108	180	328
CAL Fluor Gold 540					315	357	416	756
CAL Fluor Orange 560					210	231	315	756
CAL Fluor Red 610					336	378	504	630
Cy3	147	164	278	590	147	164	278	590
Cy5	147	164	278	590	147	164	279	590
FITC	110	124	227	459	113	123	227	459
Fluo	88	95	147	278	98	108	180	328
Hex	115	131	229	491	115	131	229	491
IRD700	192	212	378	726	192	212	378	726
IRD800	192	212	378	726	192	212	378	726
Joe	190	213	328	573	190	213	328	573
Rox	190	213	328	573	190	213	328	573
TAMRA	115	131	229	491	115	131	229	491
TET	88	95	147	278	98	108	180	328
TexasRed	297	413	508	1033	297	413	508	1033
non-Fluorescent Labels of 5' end¹
Biotin	98	121	156	393	108	131	180	444
C12 Aminolink	75	105	131	262	85	115	164	312
C6 Aminolink	33	41	49	66	49	66	82	98
Dig	247	267	323	534	262	287	363	595
Fosforylacja	49	72	98	115	59	82	115	131
inne	15	17	58	154	74	78	109	212
Methylcytosin	55	60	76	151	66	76	96	189
Tiol	161	180	213	360	174	197	247	428
fluorescent labels of 3' end¹
6-FAM					111	126	207	630
Cy3					171	207	328	670
Cy5					171	207	328	670
Fluo					111	126	171	575
Hex					192	227	287	575
TAMRA					192	227	287	575
non-Fluorescent Labels of 3' end¹
BHQ-1	101	202	302	605	101	202	302	605
BHQ-2	101	202	302	605	101	202	302	605
BHQ-3	126	252	378	756	176	252	378	756
Biotin	98	121	156	393	108	131	180	442
C7 Aminolink	39	49	98	295	49	66	131	344
Dabcyl	101	202	302	605	101	202	302	605
ddC	92	157	223	655	98	164	229	672
Deoxyuridin	25	35	45	55	45	55	66	76
Fosforylacja	50	71	91	116	60	81	101	131
inne	15	17	58	154	74	78	109	212
Inosin	25	35	45	55	45	55	66	76
Tiol	98	131	164	311	115	147	197	360
fluorescent internal modifications^{1, 2}
Cy5					277	343	504	882
Fluo					207	242	328	605
TAMRA	277	343	504	882	277	343	504	882
other internal modifications^{1, 2}
Biotin	479	529	731	1814	312	343	479	1179
BrdU	50	66	83	164	50	66	83	164
C2 AminodT					353	403	605	1462
Deoxyuridin	25	35	45	55	45	55	66	76
Inosin	35	47	61	75	61	75	89	103
Methylcytosin	55	60	76	151	60	66	81	161

¹ The price of the standard oligonucleotide will be added
² The prices for internal fluorescent modifications are valid for oligonucleotides <40 bases. For longer oligonucleotides please contact us.

Internal modification with biotin requires 1050 PLN setup fee. This modification requires a "T" to be attached.

For 5` thiol modification a set up fee of 540 PLN per order will be charged.

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Dual labelled probes

FRET (fluorescence resonance energy transfer) type hybridisation probes make possible an exceptionally sensitive detection of single gene copies. These probes are labelled at the 5'end with a fluorophore (FAM, TET, JOE, HEX, Cy3, ROX), and at the 3'end with one of Black Hole Quenchers (BHQ). BHQ molecules act as effective fluorescence quenchers in the entire visible and infrared spectrum, releasing energy mostly in the form of heat and not light. Probes based on the use of BHQs give a very low background and facilitate a very sensitive detection. Maximum of absorbance for BHQ-1 corresponds to 534 nm, and it quenches most efficiently in the range of 480-580 nm; while BHQ-2 has its maximum of absorbance at 579 nm,this makes possible fluorescence quenching between 550 and 650 nm.
Dual labelled fluorogenic probes are labelled at their 5'end with a fluorophore (FAM, TET, JOE, HEX, Fluorescein or Rhodamine), and at 3'end with a quencher (TAMRA, Dabcyl). Taqman probes (linear) are designed to quantify PCR products, detect mutations and discriminate alleles. Molecular Beacons probes (forming internal hairpins), more specific than linear probes, are used in in situ hybridisation, quantification of hybridising sequences and in allele discrimination.

Prices (PLN netto), minimum yield and offered scale of synthesis of FRET oligonucleotides and Dual labeled Fluorogenic Probes:

LP	labelling :		Reporter (nm)		Quencher Abs (nm)	cena * / skala
LP	5'	3'	Abs	Ems	Quencher Abs (nm)	5 nmol	10 nmol	20 nmol	30 nmol	50 nmol
1	6-FAM	MGB-Eclipse	495	520	522	1109	1291	1675	2434	3163
2	LC CYAN 500	BHQ-1	450	500	535	1109	1291	1675	2434	3163
3	LC CYAN 500	Eclipse	450	500	522	1109	1291	1675	2434	3163
4	LC CYAN 500	Dabcyl	450	500	453	1109	1291	1675	2434	3163
5	Fluo	TAMRA (Q)	495	520	564	451	542	706	1176	1978
6	Fluo	BHQ-1	495	520	535	451	542	706	1176	1978
7	Fluo	Eclipse	495	520	522	451	542	706	1176	1978
8	Fluo	Dabcyl	495	520	453	451	542	706	1176	1978
9	6-FAM	TAMRA (Q)	495	520	564	451	542	706	1176	1978
10	6-FAM	BHQ-1	495	520	535	451	542	706	1176	1978
11	6-FAM	Eclipse	495	520	522	451	542	706	1176	1978
12	6-FAM	Dabcyl	495	520	453	734	874	1128	1646	2146
13						0	0	0	0	0
14						0	0	0	0	0
15						0	0	0	0	0
16						0	0	0	0	0
17	TET	TAMRA (Q)	521	536	564	734	874	1128	1646	2146
18	TET	BHQ-1	521	536	535	734	874	1128	1646	2146
19	TET	Eclipse	521	536	522	734	874	1128	1646	2146
20	TET	Dabcyl	521	536	453	734	874	1128	1646	2146
21	JOE	TAMRA (Q)	522	548	564	734	874	1128	1646	2146
22	JOE	BHQ-1	522	548	535	734	874	1128	1646	2146
23	JOE	Eclipse	522	548	522	734	874	1128	1646	2146
24	JOE	Dabcyl	522	548	453	734	874	1128	1646	2146
25	YakimaYellow	TAMRA (Q)	530	549	564	734	874	1128	1646	2146
26	YakimaYellow	BHQ-1	530	549	535	734	874	1128	1646	2146
27	YakimaYellow	Eclipse	530	549	522	734	874	1128	1646	2146
28	YakimaYellow	Dabcyl	530	549	453	734	874	1128	1646	2146
29	Hex	TAMRA (Q)	535	556	564	451	542	706	1176	1978
30	Hex	BHQ-1	535	556	535	451	542	706	1176	1978
31	Hex	BHQ-2	535	556	579	451	542	706	1176	1978
32	Hex	Eclipse	535	556	522	451	542	706	1176	1978
33	Hex	Dabcyl	535	556	453	451	542	706	1176	1978
34	Cy3	BHQ-2	546	563	579	1109	1291	1675	2434	3163
35	Cy3	Eclipse	546	563	522	1109	1291	1675	2434	3163
36	TAMRA (R)	BHQ-2	564	579	579	1109	1291	1675	2434	3163
37	TAMRA (R)	Eclipse	564	579	522	1109	1291	1675	2434	3163
38	TAMRA (R)	Dabcyl	564	579	453	1109	1291	1675	2434	3163
39	ROX	TAMRA (Q)	576	601	564	1109	1291	1675	2434	3163
40	ROX	BHQ-2	576	601	579	1109	1291	1675	2434	3163
41	ROX	Eclipse	576	601	522	1109	1291	1675	2434	3163
42	Texas Red	BHQ-2	586	610	579	1109	1291	1675	2434	3163
43	Texas Red	Eclipse	586	610	522	1109	1291	1675	2434	3163
44	LC Red 610	Eclipse	590	610	522	1109	1291	1675	2434	3163
45	LC Red 610	BHQ-2	590	610	579	1109	1291	1675	2434	3163
46	LC Red 640	BHQ-2	625	640	579	1109	1291	1675	2434	3163
47	LC Red 640	BHQ-3	625	640	672	1109	1291	1675	2434	3163
48	Cy5	BHQ-2	646	662	579	1109	1291	1675	2434	3163
49	Cy5	BHQ-3	646	662	672	1109	1291	1675	2434	3163
50	Cy5.5	BHQ-2	683	705	579	1109	1291	1675	2434	3163
51	Cy5.5	BHQ-3	683	705	672	1109	1291	1675	2434	3163
52	IRD 700	BHQ-2	685	705	579	1109	1291	1675	2434	3163
53	IRD 700	BHQ-3	685	705	672	1109	1291	1675	2434	3163
54	BHQ-1	Fluo	495	520	535	451	542	706	1176	1978
55	BHQ-1	6-FAM	495	520	535	734	874	1128	1646	2146
56	BHQ-1	JOE	522	548	535	1109	1291	1675	2434	3163
57	BHQ-1	Hex	535	556	535	1109	1291	1675	2434	3163
58						0	0	0	0	0
59						0	0	0	0	0
60	BHQ-2	Hex	535	556	579	1109	1291	1675	2434	3163
61	BHQ-2	ROX	576	601	579	1109	1291	1675	2434	3163
62	BHQ-2	LC Red 610	590	610	579	1109	1291	1675	2434	3163
63	BHQ-2	LC Red 640	625	640	579	1109	1291	1675	2434	3163
64	BHQ-2	Cy5	646	662	579	1109	1291	1675	2434	3163
65	BHQ-2	Cy5.5	683	705	579	734	874	1128	1646	2146
66	Eclipse	Fluo	495	520	522	451	542	706	1176	1978
67	Eclipse	6-FAM	495	520	522	451	542	706	1176	1978
68	Eclipse	JOE	522	548	522	734	874	1128	1646	2146
69	Eclipse	Hex	535	556	522	1109	1291	1675	2434	3163
70	Eclipse	ROX	576	601	522	1109	1291	1675	2434	3163
71	Eclipse	LC Red 610	590	610	522	1109	1291	1675	2434	3163
72	Dabcyl	Fluo	495	520	453	451	542	706	1176	1978
73	Dabcyl	6-FAM	495	520	453	451	542	706	1176	1978
74	Dabcyl	JOE	522	548	453	1109	1291	1675	2434	3163
75	Fluo	BHQ-2	495	520	579	451	542	706	1176	1978
76	6-FAM	BHQ-2	495	520	579	451	542	706	1176	1978
77	TET	BHQ-2	521	536	579	734	874	1128	1646	2146
78	Cy5.5	TAMRA (Q)	683	705	564	1109	1291	1675	2434	3163
79	IRD 700	TAMRA (Q)	685	705	564	1109	1291	1675	2434	3163
80	BHQ-2	6-FAM	495	520	579	734	874	1128	1646	2146
81	BHQ-2	Cy3	546	563	579	1109	1291	1675	2434	3163
82	Cy3	BBQ-650	546	563	650	1109	1291	1675	2434	3163
83	Cy5	BBQ-650	646	662	650	1109	1291	1675	2434	3163
84	Cy5.5	BBQ-650	683	705	650	1109	1291	1675	2434	3163
85	Hex	BBQ-650	535	556	650	451	542	706	1176	1978
86	IRD 700	BBQ-650	685	705	650	1109	1291	1675	2434	3163
87	LC Red 610	BBQ-650	590	610	650	1109	1291	1675	2434	3163
88	LC Red 640	BBQ-650	625	640	650	1109	1291	1675	2434	3163
89	TAMRA (R)	BBQ-650	564	579	650	1109	1291	1675	2434	3163
90	Texas Red	BBQ-650	586	610	650	1109	1291	1675	2434	3163
91	YakimaYellow	BBQ-650	530	549	650	734	874	1128	1646	2146
92	Cy3	MGB-Eclipse	546	563	522	1109	1291	1675	2434	3163
93	Hex	MGB-Eclipse	535	556	522	1109	1291	1675	2434	3163
94	JOE	MGB-Eclipse	522	548	522	1109	1291	1675	2434	3163
95	TET	MGB-Eclipse	521	536	522	1109	1291	1675	2434	3163
96	Texas Red	MGB-Eclipse	586	610	522	1109	1291	1675	2434	3163
97	YakimaYellow	MGB-Eclipse	530	549	522	1109	1291	1675	2434	3163

* HPLC purification and MALDI ToF quality control are included.

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LightCycler Probes

The LightCycler hybridisation probe system is based on two oligonucleotide probes that hybridise, in a head-to-tail arrangement, to adjacent sequences of the target DNA. Each probe is labelled with a different fluorophore, the first one with Fluorescein at the 3'end (donor), the second one with either LC610,LC640,LC670 or LC705 at the 5'end (acceptor). Additionally, 3'OH terminus of LC probes is phosphorylated to prevent polymerase from extending the primer during PCR reaction. When the fluorophores are in close proximity, there can be a fluorescence resonance energy transfer (FRET) between them, which allows a specific and quantitative real time detection of the template amplification. To avoid sterical problems between the 3' Fluo and 5' LC labels of the probes, there should be a spacer of 1 to 5 nucleotides between the two probes.

Net prices, minimal amounts / offered scale of synthesis of LightCycler probes:

LightCycler Probes
product	price PLN netto / nmol ¹/ No. of tests²
product	1 / 300	2 / 600	3 / 1000	5 / 1500	10 / 3000	20 / 6000	30 / 10000
5´LC610/640/670-3´Fosforan	819	924	1050	1575	2058	3318	3864
3´LC610/640	819	924	1050	1575	2058	3318	3864
wew.LC610/640	1050	1176	1470	1995	2478	4158	5838
5´LC705-3´Fosforan	819	924	1050	1575	2058	3318	3864
3´-Fluoresceina	336	366	399	483	1050	1302	1575

¹ For oligonucleotides > 33 bases price for each extra base is 20 PLN.
² Values calculated for 3 pmol of probe per assay (range of optimal amount 2-10 pmol).
* Price includes HPLC purification and MALDI ToF quality check to provide probes of exceptional purity.
* LightCycler® is a trademark of Idaho Technology Inc. Custom synthesis of LC probes and oligonucleotides containing LCRed dyes under licence of Roche Diagnostics.
* Sold under license from Hoffmann La-Roche Ltd. All products are for research only.

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RNA oligonucleotides

Our offer includes the synthesis of labelled and standard RNA oligos.
We have available many types of modifications, both at the 5 ', 3' ends and internal. Please contact us for specific information and pricing for individual RNA modifications.

RNA oligonucleotides are HPLC purified.

Offered delivered quantities and the prices of RNA oligonucleotides synthesis up to 40nt:

RNA oligonucleotides
synthesis scale [umol]	price PLN netto
synthesis scale [umol]	price for oligonucleotide ≤ 10 nt	price for base >10 nt ≥ 40 nt
5-25	237	21
25-50	316	28
50-100	356	32
100-200	455	41
200-400	595	55

* RNA oligonucleotides longer than 40nt - please contact us
* A higher scale of synthesis - please contact us

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