Next generation sequencing services
DNA Sequence Analysis
Services offered:
Assembly of single readings into longer sequences. Creation of single contigs.
Primer design and set-up of PCR and sequencing conditions.
Restriction analysis of DNA fragments,creation of restriction maps.
Search for open reading frames (ORFs) and ORF analysis; identification of protein motifs and cellular localisation signals.
Search for homologous sequences and creation of phylogenetic trees
Mutation analysis, comparison of the given sequence with the reference one.
Useful Links:
Align two sequences using BLAST (bl2seq)
Nucleotide blast
Oligo Calc: Oligonucleotide Properties Calculator
Reverse Complement
Pick primers from a DNA sequence : Primer 3.0
UCSC In-Silico PCR
Others:
Calculator : DNA concentration -> number of molecules per microliter