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Nauka > Oligonucleotides > Offer and prices
Offer and prices    

We offer the synthesis of DNA and RNA oligos, fluorescent and other oligonucleotide modifications as well as purification using the HPLC method.

DNA oligonucleotides:

RNA oligonucleotides:


deoxynucleotides

  • mi-N1005S dNTP Set 100 mM (in water, 4x 250 µl) - 390 PLN net price
  • mi-N1006M dNTP Mix 10 mM (in water, 250 µl) - 110 PLN net price
Delivery:

DNA and RNA oligos are delivered in a lyophylised form along with an information concerning the quantity in which a given lyophylisate should be resuspended to obtain 100 µM; concentration.
We advise 10 mM Tris pH 7.5. when resuspending DNA oligos.

Orders:

Please order online by filling out the form. If you encounter any problem while ordering, please do it by e-mail.

Order processing time:
  • standard oligonucleotides: 2-3 working days.
  • standard oligonucleotides with HPLC purification: 5-10 working days.
  • labelled oligonucleotides: 9-15 working days.
  • RNA oligonucleotides: please contact us
Delivery costs:
  • When your orders value is greater than 200 PLN Genomed covers the costs of delivery mail in Poland.
  • Orders for less than 200 PLN net - delivery cost in Poland - 25 PLN netto
  • Primers can also be shipped by registered letter free of charge.
Prices:

Prices are listed in the tables below. With the help of the oligonucleotides ordering form it is possible to calculate the cost of an oligonucleotide.

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standard oligonucleotides


Net prices (PLN), minimum yield and offered synthesis scale of standard oligonucleotides:

 standard oligonucleotides
synthesis scale [umol]
(µmol)
purification standard 1purification HPLC
price per base 2
(netto PLN)
minimum yield
OD (260)3
price per base
(netto PLN)
minimum yield
OD (260)
0.0241.133.451
0.041.3 53.652.5
0.202.5 1658
1.009.9 801225

1 Standard desalting consists in ethanol precipitation. It guarantees a level of purification sufficient for standard applications (PCR reaction and sequencing)
2 For oligonucleotides longer than 80 bp price is 110% higher. For oligonucleotides longer than 90 bp price is 120% higher. For oligonucleotides shorter than 15 bp price calculated as for 15-mer..
3 Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed.
4 0.02 µmol scale only for oligos up to 33 bases.
A higher scale of synthesis - please contact us
Wobble - zasada zdegenerowana na 3' końcu - 54 PLN

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antisense oligonucleotides (PTOs)


Net prices (PLN), minimum yield and offered synthesis scale of phosphorothioates (PTOs, S-oligos).
Prices include HPLC- and cartridge-purification, if the oligo is a "Full-PTO"!
For mixed Phosphosphorothioate/Phosphorodiester-Oligos (i. e. "end capping") cartridge-purification will be charged at 65 PLN net price. We recommend this service

 antisense oligonucleotides (PTOs)
synthesis scale [umol]
(µmol)
purification HPLC
price per base
(netto PLN)
minimum yield
OD (260)1
0.2013 5
1.0027 15
10.009280

1 Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed.
A higher scale of synthesis - please contact us

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labelled oligonucleotides


Genomed offers labelling of oligos at 5' and 3' ends as well as internal modifications. If the type of labelling you are interested in is not to be found on the price list, please enquire.

Synthesis of labelled oligonucleotides, offered scales, minmal amount and net prices:

 fluorescent labels of 5' end1
purification:standardHPLC
synthesis scale [umol] (µmol):
minimum yield (OD260):
0.02
3
0.04
5
0.20
12
1.00
50
0.02
1
0.04
2
0.20
5
1.00
12
6-FAM889514727898108180328
CAL Fluor Gold 540315357416756
CAL Fluor Orange 560210231315756
CAL Fluor Red 610336378504630
Cy3147164278590147164278590
Cy5147164278590147164279590
FITC110124227459113123227459
Fluo889514727898108180328
Hex115131229491115131229491
IRD700192212378726192212378726
IRD800192212378726192212378726
Joe190213328573190213328573
Rox190213328573190213328573
TAMRA115131229491115131229491
TET889514727898108180328
TexasRed29741350810332974135081033
 non-Fluorescent Labels of 5' end1
Biotin98121156393108131180444
C12 Aminolink7510513126285115164312
C6 Aminolink3341496649668298
Dig247267323534262287363595
Fosforylacja4972981155982115131
inne1517581547478109212
Methylcytosin556076151667696189
Tiol161180213360174197247428
 fluorescent labels of 3' end1
6-FAM111126207630
Cy3171207328670
Cy5171207328670
Fluo111126171575
Hex192227287575
TAMRA192227287575
 non-Fluorescent Labels of 3' end1
BHQ-1101202302605101202302605
BHQ-2101202302605101202302605
BHQ-3126252378756176252378756
Biotin98121156393108131180442
C7 Aminolink3949982954966131344
Dabcyl101202302605101202302605
ddC9215722365598164229672
Deoxyuridin2535455545556676
Fosforylacja5071911166081101131
inne1517581547478109212
Inosin2535455545556676
Tiol98131164311115147197360
 fluorescent internal modifications1, 2
Cy5277343504882
Fluo207242328605
TAMRA277343504882277343504882
 other internal modifications1, 2
Biotin47952973118143123434791179
BrdU506683164506683164
C2 AminodT3534036051462
Deoxyuridin2535455545556676
Inosin2535455545556676
Methylcytosin556076151606681161

1 The price of the standard oligonucleotide will be added
2 The prices for internal fluorescent modifications are valid for oligonucleotides <40 bases. For longer oligonucleotides please contact us.

Internal modification with biotin requires 1050 PLN setup fee. This modification requires a "T" to be attached.

For 5` thiol modification a set up fee of 540 PLN per order will be charged.

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Dual labelled probes


  • FRET (fluorescence resonance energy transfer) type hybridisation probes make possible an exceptionally sensitive detection of single gene copies. These probes are labelled at the 5'end with a fluorophore (FAM, TET, JOE, HEX, Cy3, ROX), and at the 3'end with one of Black Hole Quenchers (BHQ). BHQ molecules act as effective fluorescence quenchers in the entire visible and infrared spectrum, releasing energy mostly in the form of heat and not light. Probes based on the use of BHQs give a very low background and facilitate a very sensitive detection. Maximum of absorbance for BHQ-1 corresponds to 534 nm, and it quenches most efficiently in the range of 480-580 nm; while BHQ-2 has its maximum of absorbance at 579 nm,this makes possible fluorescence quenching between 550 and 650 nm.

  • Dual labelled fluorogenic probes are labelled at their 5'end with a fluorophore (FAM, TET, JOE, HEX, Fluorescein or Rhodamine), and at 3'end with a quencher (TAMRA, Dabcyl). Taqman probes (linear) are designed to quantify PCR products, detect mutations and discriminate alleles. Molecular Beacons probes (forming internal hairpins), more specific than linear probes, are used in in situ hybridisation, quantification of hybridising sequences and in allele discrimination.

Prices (PLN netto), minimum yield and offered scale of synthesis of FRET oligonucleotides and Dual labeled Fluorogenic Probes:

LPlabelling : Reporter (nm)Quencher
Abs (nm)
cena * / skala
5'3'AbsEms 5 nmol10 nmol20 nmol30 nmol50 nmol
16-FAM MGB-Eclipse 495 520 522 10131205155022562942
2LC CYAN 500 BHQ-1 450 500 535 10131205155022562942
3LC CYAN 500 Eclipse 450 500 522 10131205155022562942
4LC CYAN 500 Dabcyl 450 500 453 10131205155022562942
5Fluo TAMRA (Q) 495 520 564 45154270611761978
6Fluo BHQ-1 495 520 535 45154270611761978
7Fluo Eclipse 495 520 522 45154270611761978
8Fluo Dabcyl 495 520 453 45154270611761978
96-FAM TAMRA (Q) 495 520 564 45154270611761978
106-FAM BHQ-1 495 520 535 45154270611761978
116-FAM Eclipse 495 520 522 45154270611761978
126-FAM Dabcyl 495 520 453 734874112816462146
13 00000
14 00000
15 00000
16 00000
17TET TAMRA (Q) 521 536 564 734874112816462146
18TET BHQ-1 521 536 535 734874112816462146
19TET Eclipse 521 536 522 734874112816462146
20TET Dabcyl 521 536 453 734874112816462146
21JOE TAMRA (Q) 522 548 564 734874112816462146
22JOE BHQ-1 522 548 535 734874112816462146
23JOE Eclipse 522 548 522 734874112816462146
24JOE Dabcyl 522 548 453 734874112816462146
25YakimaYellow TAMRA (Q) 530 549 564 734874112816462146
26YakimaYellow BHQ-1 530 549 535 734874112816462146
27YakimaYellow Eclipse 530 549 522 734874112816462146
28YakimaYellow Dabcyl 530 549 453 734874112816462146
29Hex TAMRA (Q) 535 556 564 45154270611761978
30Hex BHQ-1 535 556 535 45154270611761978
31Hex BHQ-2 535 556 579 45154270611761978
32Hex Eclipse 535 556 522 45154270611761978
33Hex Dabcyl 535 556 453 45154270611761978
34Cy3 BHQ-2 546 563 579 10131205155022562942
35Cy3 Eclipse 546 563 522 10131205155022562942
36TAMRA (R) BHQ-2 564 579 579 10131205155022562942
37TAMRA (R) Eclipse 564 579 522 10131205155022562942
38TAMRA (R) Dabcyl 564 579 453 10131205155022562942
39ROX TAMRA (Q) 576 601 564 10131205155022562942
40ROX BHQ-2 576 601 579 10131205155022562942
41ROX Eclipse 576 601 522 10131205155022562942
42Texas Red BHQ-2 586 610 579 10131205155022562942
43Texas Red Eclipse 586 610 522 10131205155022562942
44LC Red 610 Eclipse 590 610 522 10131205155022562942
45LC Red 610 BHQ-2 590 610 579 10131205155022562942
46LC Red 640 BHQ-2 625 640 579 10131205155022562942
47LC Red 640 BHQ-3 625 640 672 10131205155022562942
48Cy5 BHQ-2 646 662 579 10131205155022562942
49Cy5 BHQ-3 646 662 672 10131205155022562942
50Cy5.5 BHQ-2 683 705 579 10131205155022562942
51Cy5.5 BHQ-3 683 705 672 10131205155022562942
52IRD 700 BHQ-2 685 705 579 10131205155022562942
53IRD 700 BHQ-3 685 705 672 10131205155022562942
54BHQ-1 Fluo 495 520 535 45154270611761978
55BHQ-1 6-FAM 495 520 535 734874112816462146
56BHQ-1 JOE 522 548 535 10131205155022562942
57BHQ-1 Hex 535 556 535 10131205155022562942
58 00000
59 00000
60BHQ-2 Hex 535 556 579 10131205155022562942
61BHQ-2 ROX 576 601 579 10131205155022562942
62BHQ-2 LC Red 610 590 610 579 10131205155022562942
63BHQ-2 LC Red 640 625 640 579 10131205155022562942
64BHQ-2 Cy5 646 662 579 10131205155022562942
65BHQ-2 Cy5.5 683 705 579 734874112816462146
66Eclipse Fluo 495 520 522 45154270611761978
67Eclipse 6-FAM 495 520 522 45154270611761978
68Eclipse JOE 522 548 522 734874112816462146
69Eclipse Hex 535 556 522 10131205155022562942
70Eclipse ROX 576 601 522 10131205155022562942
71Eclipse LC Red 610 590 610 522 10131205155022562942
72Dabcyl Fluo 495 520 453 45154270611761978
73Dabcyl 6-FAM 495 520 453 45154270611761978
74Dabcyl JOE 522 548 453 10131205155022562942
75Fluo BHQ-2 495 520 579 45154270611761978
766-FAM BHQ-2 495 520 579 45154270611761978
77TET BHQ-2 521 536 579 734874112816462146
78Cy5.5 TAMRA (Q) 683 705 564 10131205155022562942
79IRD 700 TAMRA (Q) 685 705 564 10131205155022562942
80BHQ-2 6-FAM 495 520 579 734874112816462146
81BHQ-2 Cy3 546 563 579 10131205155022562942
82Cy3 BBQ-650 546 563 650 10131205155022562942
83Cy5 BBQ-650 646 662 650 10131205155022562942
84Cy5.5 BBQ-650 683 705 650 10131205155022562942
85Hex BBQ-650 535 556 650 45154270611761978
86IRD 700 BBQ-650 685 705 650 10131205155022562942
87LC Red 610 BBQ-650 590 610 650 10131205155022562942
88LC Red 640 BBQ-650 625 640 650 10131205155022562942
89TAMRA (R) BBQ-650 564 579 650 10131205155022562942
90Texas Red BBQ-650 586 610 650 10131205155022562942
91YakimaYellow BBQ-650 530 549 650 734874112816462146
92Cy3 MGB-Eclipse 546 563 522 10131205155022562942
93Hex MGB-Eclipse 535 556 522 10131205155022562942
94JOE MGB-Eclipse 522 548 522 10131205155022562942
95TET MGB-Eclipse 521 536 522 10131205155022562942
96Texas Red MGB-Eclipse 586 610 522 10131205155022562942
97YakimaYellow MGB-Eclipse 530 549 522 10131205155022562942
* HPLC purification and MALDI ToF quality control are included.

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LightCycler Probes


The LightCycler hybridisation probe system is based on two oligonucleotide probes that hybridise, in a head-to-tail arrangement, to adjacent sequences of the target DNA. Each probe is labelled with a different fluorophore, the first one with Fluorescein at the 3'end (donor), the second one with either LC610,LC640,LC670 or LC705 at the 5'end (acceptor). Additionally, 3'OH terminus of LC probes is phosphorylated to prevent polymerase from extending the primer during PCR reaction. When the fluorophores are in close proximity, there can be a fluorescence resonance energy transfer (FRET) between them, which allows a specific and quantitative real time detection of the template amplification. To avoid sterical problems between the 3' Fluo and 5' LC labels of the probes, there should be a spacer of 1 to 5 nucleotides between the two probes.

Net prices, minimal amounts / offered scale of synthesis of LightCycler probes:

 LightCycler Probes
productprice PLN netto / nmol 1/ No. of tests2
1 / 3002 / 6003 / 10005 / 150010 / 300020 / 600030 / 10000
5´LC610/640/670-3´Fosforan81992410501575205833183864
3´LC610/64081992410501575205833183864
wew.LC610/6401050117614701995247841585838
5´LC705-3´Fosforan81992410501575205833183864
3´-Fluoresceina336366399483105013021575

1 For oligonucleotides > 33 bases price for each extra base is 20 PLN.
2 Values calculated for 3 pmol of probe per assay (range of optimal amount 2-10 pmol).
* Price includes HPLC purification and MALDI ToF quality check to provide probes of exceptional purity.
* LightCycler® is a trademark of Idaho Technology Inc. Custom synthesis of LC probes and oligonucleotides containing LCRed dyes under licence of Roche Diagnostics.
* Sold under license from Hoffmann La-Roche Ltd. All products are for research only.

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RNA oligonucleotides


Our offer includes the synthesis of labelled and standard RNA oligos.
We have available many types of modifications, both at the 5 ', 3' ends and internal. Please contact us for specific information and pricing for individual RNA modifications.

RNA oligonucleotides are HPLC purified.

Offered delivered quantities and the prices of RNA oligonucleotides synthesis up to 40nt:

 RNA oligonucleotides
synthesis scale [umol]price PLN netto
price for oligonucleotide ≤ 10 ntprice for base >10 nt ≥ 40 nt
5-2523721
25-5031628
50-10035632
100-20045541
200-40059555


* RNA oligonucleotides longer than 40nt - please contact us
* A higher scale of synthesis - please contact us

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