We offer the synthesis of DNA and RNA oligos, fluorescent and other oligonucleotide modifications as well as purification using the HPLC method. DNA oligonucleotides:
- standard oligonucleotides
synthesis scale [umol]
(µmol) | purification standard 1 | purification HPLC |
|---|
price per base 2
(netto PLN) | minimum yield
OD (260)3 | price per base
(netto PLN) | minimum yield
OD (260) |
|---|
| 0.024 | 1.1 | 3 | 3.45 | 1 | | 0.04 | 1.3 | 5 | 3.65 | 2.5 | | 0.20 | 2.5 | 16 | 5 | 8 | | 1.00 | 9.9 | 80 | 12 | 25 |
1 Standard desalting consists in ethanol precipitation. It guarantees a level of purification sufficient for standard applications (PCR reaction and sequencing)
2 For oligonucleotides longer than 80 bp price is 110% higher. For oligonucleotides longer than 90 bp price is 120% higher. For oligonucleotides shorter than 15 bp price calculated as for 15-mer..
3 Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed.
4 0.02 µmol scale only for oligos up to 33 bases.
- antisense oligonucleotides (PTOs)
- labelled oligonucleotides
RNA oligonucleotides:
Delivery:
DNA and RNA oligos are delivered in a lyophylised form along with an information concerning the quantity in which a given lyophylisate should be resuspended to obtain 100 µM; concentration.
We advise 10 mM Tris pH 7.5. when resuspending DNA oligos.
Orders:
Please order online by filling out the form. If you encounter any problem while ordering, please do it by e-mail.
Order processing time:
- standard oligonucleotides: 2-3 working days.
- standard oligonucleotides with HPLC purification: do 5 working days.
- labelled oligonucleotides: do 10 working days.
- RNA oligonucleotides: please contact us
Delivery costs:
- When your orders value is greater than 200 PLN Genomed covers the costs of delivery mail in Poland.
- Orders for less than 200 PLN net - delivery cost in Poland - 30 PLN netto
- Primers can also be shipped by registered letter free of charge.
Prices:
Prices are listed in the tables below. With the help of the oligonucleotides ordering form it is possible to calculate the cost of an oligonucleotide.
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standard oligonucleotides
Net prices (PLN), minimum yield and offered synthesis scale of standard oligonucleotides:
| standard oligonucleotides |
|---|
synthesis scale [umol]
(µmol) | purification standard 1 | purification HPLC |
|---|
price per base 2
(netto PLN) | minimum yield
OD (260)3 | price per base
(netto PLN) | minimum yield
OD (260) |
|---|
| 0.024 | 1.1 | 3 | 3.45 | 1 | | 0.04 | 1.3 | 5 | 3.65 | 2.5 | | 0.20 | 2.5 | 16 | 5 | 8 | | 1.00 | 9.9 | 80 | 12 | 25 |
1 Standard desalting consists in ethanol precipitation. It guarantees a level of purification sufficient for standard applications (PCR reaction and sequencing)
2 For oligonucleotides longer than 80 bp price is 110% higher. For oligonucleotides longer than 90 bp price is 120% higher. For oligonucleotides shorter than 15 bp price calculated as for 15-mer..
3 Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed.
4 0.02 µmol scale only for oligos up to 33 bases.
A higher scale of synthesis - please contact us
Wobble - zasada zdegenerowana na 3' końcu - 54 PLNtop of the page
antisense oligonucleotides (PTOs)
Net prices (PLN), minimum yield and offered synthesis scale of phosphorothioates (PTOs, S-oligos).
Prices include HPLC- and cartridge-purification, if the oligo is a "Full-PTO"!
For mixed Phosphosphorothioate/Phosphorodiester-Oligos (i. e. "end capping") cartridge-purification will be charged at 65 PLN net price. We recommend this service
| antisense oligonucleotides (PTOs) |
|---|
synthesis scale [umol]
(µmol) | purification HPLC |
|---|
price per base
(netto PLN) | minimum yield
OD (260)1 |
|---|
| 0.20 | 13 | 5 | | 1.00 | 27 | 15 | | 10.00 | 92 | 80 |
1 Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed.
A higher scale of synthesis - please contact us
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labelled oligonucleotides
Genomed offers labelling of oligos at 5' and 3' ends as well as internal modifications. If the type of labelling you are interested in is not to be found on the price list, please enquire.
Synthesis of labelled oligonucleotides, offered scales, minmal amount and net prices:
| fluorescent labels of 5' end1 |
|---|
| purification: | standard | HPLC |
|---|
synthesis scale [umol] (µmol):
minimum yield (OD260): | 0.02
3 | 0.04
5 | 0.20
12 | 1.00
50 | 0.02
1 | 0.04
2 | 0.20
5 | 1.00
12 |
|---|
| 6-FAM | 88 | 95 | 147 | 278 | 98 | 108 | 180 | 328 | | CAL Fluor Gold 540 | | | | | 315 | 357 | 416 | 756 | | CAL Fluor Orange 560 | | | | | 210 | 231 | 315 | 756 | | CAL Fluor Red 610 | | | | | 336 | 378 | 504 | 630 | | Cy3 | 147 | 164 | 278 | 590 | 147 | 164 | 278 | 590 | | Cy5 | 147 | 164 | 278 | 590 | 147 | 164 | 279 | 590 | | FITC | 110 | 124 | 227 | 459 | 113 | 123 | 227 | 459 | | Fluo | 88 | 95 | 147 | 278 | 98 | 108 | 180 | 328 | | Hex | 115 | 131 | 229 | 491 | 115 | 131 | 229 | 491 | | IRD700 | 192 | 212 | 378 | 726 | 192 | 212 | 378 | 726 | | IRD800 | 192 | 212 | 378 | 726 | 192 | 212 | 378 | 726 | | Joe | 190 | 213 | 328 | 573 | 190 | 213 | 328 | 573 | | Rox | 190 | 213 | 328 | 573 | 190 | 213 | 328 | 573 | | TAMRA | 115 | 131 | 229 | 491 | 115 | 131 | 229 | 491 | | TET | 88 | 95 | 147 | 278 | 98 | 108 | 180 | 328 | | TexasRed | 297 | 413 | 508 | 1033 | 297 | 413 | 508 | 1033 | | non-Fluorescent Labels of 5' end1 |
|---|
| Biotin | 98 | 121 | 156 | 393 | 108 | 131 | 180 | 444 | | C12 Aminolink | 75 | 105 | 131 | 262 | 85 | 115 | 164 | 312 | | C6 Aminolink | 33 | 41 | 49 | 66 | 49 | 66 | 82 | 98 | | Dig | 247 | 267 | 323 | 534 | 262 | 287 | 363 | 595 | | Fosforylacja | 49 | 72 | 98 | 115 | 59 | 82 | 115 | 131 | | inne | 15 | 17 | 58 | 154 | 74 | 78 | 109 | 212 | | Methylcytosin | 55 | 60 | 76 | 151 | 66 | 76 | 96 | 189 | | Tiol | 161 | 180 | 213 | 360 | 174 | 197 | 247 | 428 | | fluorescent labels of 3' end1 |
|---|
| 6-FAM | | | | | 111 | 126 | 207 | 630 | | Cy3 | | | | | 171 | 207 | 328 | 670 | | Cy5 | | | | | 171 | 207 | 328 | 670 | | Fluo | | | | | 111 | 126 | 171 | 575 | | Hex | | | | | 192 | 227 | 287 | 575 | | TAMRA | | | | | 192 | 227 | 287 | 575 | | non-Fluorescent Labels of 3' end1 |
|---|
| BHQ-1 | 101 | 202 | 302 | 605 | 101 | 202 | 302 | 605 | | BHQ-2 | 101 | 202 | 302 | 605 | 101 | 202 | 302 | 605 | | BHQ-3 | 126 | 252 | 378 | 756 | 176 | 252 | 378 | 756 | | Biotin | 98 | 121 | 156 | 393 | 108 | 131 | 180 | 442 | | C7 Aminolink | 39 | 49 | 98 | 295 | 49 | 66 | 131 | 344 | | Dabcyl | 101 | 202 | 302 | 605 | 101 | 202 | 302 | 605 | | ddC | 92 | 157 | 223 | 655 | 98 | 164 | 229 | 672 | | Deoxyuridin | 25 | 35 | 45 | 55 | 45 | 55 | 66 | 76 | | Fosforylacja | 50 | 71 | 91 | 116 | 60 | 81 | 101 | 131 | | inne | 15 | 17 | 58 | 154 | 74 | 78 | 109 | 212 | | Inosin | 25 | 35 | 45 | 55 | 45 | 55 | 66 | 76 | | Tiol | 98 | 131 | 164 | 311 | 115 | 147 | 197 | 360 | | fluorescent internal modifications1, 2 |
|---|
| Cy5 | | | | | 277 | 343 | 504 | 882 | | Fluo | | | | | 207 | 242 | 328 | 605 | | TAMRA | 277 | 343 | 504 | 882 | 277 | 343 | 504 | 882 | | other internal modifications1, 2 |
|---|
| Biotin | 479 | 529 | 731 | 1814 | 312 | 343 | 479 | 1179 | | BrdU | 50 | 66 | 83 | 164 | 50 | 66 | 83 | 164 | | C2 AminodT | | | | | 353 | 403 | 605 | 1462 | | Deoxyuridin | 25 | 35 | 45 | 55 | 45 | 55 | 66 | 76 | | Inosin | 35 | 47 | 61 | 75 | 61 | 75 | 89 | 103 | | Methylcytosin | 55 | 60 | 76 | 151 | 60 | 66 | 81 | 161 |
1 The price of the standard oligonucleotide will be added
2 The prices for internal fluorescent modifications are valid for oligonucleotides <40 bases. For longer oligonucleotides please contact us.
Internal modification with biotin requires 1050 PLN setup fee.
This modification requires a "T" to be attached.
For 5` thiol modification a set up fee of 540 PLN per order will be charged.
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Dual labelled probes
- FRET (fluorescence resonance energy transfer) type hybridisation probes
make possible an exceptionally sensitive detection of single gene copies.
These probes are labelled at the 5'end with a fluorophore
(FAM, TET, JOE, HEX, Cy3, ROX), and at the 3'end with one of
Black Hole Quenchers (BHQ). BHQ molecules act as effective fluorescence
quenchers in the entire visible and infrared spectrum, releasing energy mostly
in the form of heat and not light. Probes based on the use of
BHQs give a very low background and facilitate a very sensitive detection.
Maximum of absorbance for BHQ-1 corresponds to 534 nm, and it quenches most efficiently in the range
of 480-580 nm; while BHQ-2 has its maximum of absorbance at 579 nm,this makes possible
fluorescence quenching between 550 and 650 nm.
- Dual labelled fluorogenic probes are labelled at their 5'end with a fluorophore (FAM,
TET, JOE, HEX, Fluorescein or Rhodamine), and at 3'end with
a quencher (TAMRA, Dabcyl). Taqman probes (linear) are designed to quantify PCR products, detect mutations and discriminate alleles.
Molecular Beacons probes (forming internal hairpins), more specific than linear probes, are used in in situ hybridisation, quantification of hybridising sequences and in allele discrimination.
Prices (PLN netto), minimum yield and offered scale of synthesis of FRET oligonucleotides and Dual labeled Fluorogenic Probes:
* HPLC purification and MALDI ToF quality control are included.top of the page
LightCycler Probes
The LightCycler hybridisation probe system is based on two oligonucleotide probes
that hybridise, in a head-to-tail arrangement, to adjacent sequences of the target DNA.
Each probe is labelled with a different fluorophore, the first one with Fluorescein at the 3'end (donor),
the second one with either LC610,LC640,LC670 or LC705 at the 5'end (acceptor).
Additionally, 3'OH terminus of LC probes is phosphorylated to prevent polymerase from extending the primer
during PCR reaction. When the fluorophores are in close proximity, there can be a fluorescence resonance
energy transfer (FRET) between them, which allows a specific and quantitative real time detection of the
template amplification. To avoid sterical problems between the 3' Fluo and 5' LC labels of
the probes, there should be a spacer of 1 to 5 nucleotides between the two probes.
Net prices, minimal amounts / offered scale of synthesis of LightCycler probes:
| LightCycler Probes | | product | price PLN netto / nmol 1/ No. of tests2 |
|---|
| 1 / 300 | 2 / 600 | 3 / 1000 | 5 / 1500 | 10 / 3000 | 20 / 6000 | 30 / 10000 |
|---|
| 5´LC610/640/670-3´Fosforan | 819 | 924 | 1050 | 1575 | 2058 | 3318 | 3864 | | 3´LC610/640 | 819 | 924 | 1050 | 1575 | 2058 | 3318 | 3864 | | wew.LC610/640 | 1050 | 1176 | 1470 | 1995 | 2478 | 4158 | 5838 | | 5´LC705-3´Fosforan | 819 | 924 | 1050 | 1575 | 2058 | 3318 | 3864 | | 3´-Fluoresceina | 336 | 366 | 399 | 483 | 1050 | 1302 | 1575 |
1 For oligonucleotides > 33 bases price for each extra base is 20 PLN.
2 Values calculated for 3 pmol of probe per assay (range of optimal amount 2-10 pmol).
* Price includes HPLC purification and MALDI ToF quality check to provide probes of exceptional purity.
* LightCycler® is a trademark of Idaho Technology Inc. Custom synthesis of LC probes and oligonucleotides containing LCRed dyes under licence of Roche Diagnostics.
* Sold under license from Hoffmann La-Roche Ltd. All products are for research only.top of the page
RNA oligonucleotides
Our offer includes the synthesis of labelled and standard RNA oligos.
We have available many types of modifications, both at the 5 ', 3' ends and internal. Please contact us for specific information and pricing for individual RNA modifications.
RNA oligonucleotides are HPLC purified.
Offered delivered quantities and the prices of RNA oligonucleotides synthesis up to 40nt:
| RNA oligonucleotides |
|---|
| synthesis scale [umol] | price PLN netto |
|---|
| price for oligonucleotide ≤ 10 nt | price for base >10 nt ≥ 40 nt |
|---|
| 5-25 | 237 | 21 | | 25-50 | 316 | 28 | | 50-100 | 356 | 32 | | 100-200 | 455 | 41 | | 200-400 | 595 | 55 |
* RNA oligonucleotides longer than 40nt - please contact us
* A higher scale of synthesis - please contact ustop of the page
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Używamy