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Nauka > Oligonucleotides > Offer and prices
Offer and prices    

We offer the synthesis of DNA and RNA oligos, fluorescent and other oligonucleotide modifications as well as purification using the HPLC method.

DNA oligonucleotides:

RNA oligonucleotides:


peptides


deoxynucleotides

  • mi-N1005S dNTP Set 100 mM (in water, 4x 250 µl) - 390 PLN net price
  • mi-N1006M dNTP Mix 10 mM (in water, 250 µl) - 110 PLN net price
Delivery:

Peptides and both DNA and RNA oligos are delivered in a lyophylised form along with an information concerning the quantity in which a given lyophylisate should be resuspended to obtain 100 µM; concentration.
We advise 10 mM Tris pH 7.5. when resuspending DNA oligos.

Orders:

Please order online by filling out the form. If you encounter any problem while ordering, please do it by e-mail.

Order processing time:
  • standard oligonucleotides: 2-3 working days.
  • standard oligonucleotides with HPLC purification: 3-5 working days.
  • labelled oligonucleotides: 5-9 working days.
  • RNA oligonucleotides & peptides: please contact us
  • RNA oligonucleotides & peptides: kontakt
Delivery costs:
  • When your order's value is greater than 180 PLN Genomed covers the costs of delivery mail in Poland.
  • Orders for less than 150 PLN net - delivery cost in POland - 20 PLN netto
  • Primers can also be shipped by registered letter free of charge.
Prices:

Prices are listed in the tables below. With the help of the oligonucleotides ordering form it is possible to calculate the cost of an oligonucleotide.

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standard oligonucleotides


Net prices (PLN), minimum yield and offered synthesis scale of standard oligonucleotides:

 standard oligonucleotides
scale
(µmol)
purification standard 1purification HPLC
price per base 2
(netto PLN)
minimum yield
OD (260)3
price per base
(netto PLN)
minimum yield
OD (260)
0.0241331
0.041.1 53.22.5
0.202.0 1648
1.006.5 808.525

1 Standard desalting consists in ethanol precipitation. It guarantees a level of purification sufficient for standard applications (PCR reaction and sequencing)
2 For oligonucleotides longer than 80 bp price is 110% higher. For oligonucleotides longer than 90 bp price is 120% higher. For oligonucleotides shorter than 15 bp price calculated as for 15-mer..
3 Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed.
4 0.02 µmol scale only for oligos up to 33 bases.
A higher scale of synthesis - please contact us
Wobble - zasada zdegenerowana na 3' końcu - 54 PLN

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antisense oligonucleotides (PTOs)


Net prices (PLN), minimum yield and offered synthesis scale of phosphorothioates (PTOs, S-oligos).
Prices include HPLC- and cartridge-purification, if the oligo is a "Full-PTO"!
For mixed Phosphosphorothioate/Phosphorodiester-Oligos (i. e. "end capping") cartridge-purification will be charged at 65 PLN net price. We recommend this service

 antisense oligonucleotides (PTOs)
scale
(µmol)
purification HPLC
price per base
(netto PLN)
minimum yield
OD (260)1
0.2013 5
1.0027 15
10.009280

1 Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed.
A higher scale of synthesis - please contact us

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labelled oligonucleotides


Genomed offers labelling of oligos at 5' and 3' ends as well as internal modifications. If the type of labelling you are interested in is not to be found on the price list, please enquire.

Synthesis of labelled oligonucleotides, offered scales, minmal amount and net prices:

 fluorescent labels of 5' end1
purification:standardHPLC
scale (µmol):
minimum yield (OD260):
0.02
3
0.04
5
0.20
12
1.00
50
0.02
1
0.04
2
0.20
5
1.00
12
6-FAM100110158315110121189368
CAL Fluor Gold 540315357416756
CAL Fluor Orange 560210231315756
CAL Fluor Red 610336378504630
Cy3184205341730
Cy5184205341730
FITC106118200383
Fluo110120179341120131221399
Hex143162286617
IRD700173191362751
IRD800173191362751
Joe236268399704
Rox236268399704
TAMRA143162286617
TET110120179341120131221399
TexasRed60466276712186206838091302
 non-Fluorescent Labels of 5' end1
Biotin126155197504139168231546
C12 Aminolink89126158315100137200378
C6 Aminolink3747791266384131168
Dig252273336578273294368651
Fosforylacja588411613774100137163
inne11111111
Methylcytosin6371841687484105210
Tiol78379884011557908198821155
 fluorescent labels of 3' end1
6-FAM126137231840
Cy3189231357798
Cy5189231357798
Fluo126139189630
Hex2102524831680
TAMRA210252315924
 non-Fluorescent Labels of 3' end1
BHQ-12313154831491
BHQ-22733575671659
BHQ-33153995881680
Biotin126155197504139168231546
C7 Aminolink50631263786384168441
Dabcyl210294399819
ddC118202286840126210294861
Deoxyuridin3242506353637484
Fosforylacja639212614776105147168
inne11111111
Inosin3242506353637484
Tiol126168210399147189252462
 fluorescent internal modifications1, 2
Cy56097989561628
Fluo63084010081680
TAMRA63084010081680
 other internal modifications1, 2
Biotin3784205881470
BrdU189210231168200252294588
C2 AminodT2843154831176
Deoxyuridin2938465850596879
Inosin2938465850596879
Methylcytosin6167791587180100200

1 The price of the standard oligonucleotide will be added
2 The prices for internal fluorescent modifications are valid for oligonucleotides <40 bases. For longer oligonucleotides please contact us.

Internal modification with biotin requires 1050 PLN setup fee. This modification requires a "T" to be attached.

For 5` thiol modification a set up fee of 540 PLN per order will be charged.

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Dual labelled probes


  • FRET (fluorescence resonance energy transfer) type hybridisation probes make possible an exceptionally sensitive detection of single gene copies. These probes are labelled at the 5'end with a fluorophore (FAM, TET, JOE, HEX, Cy3, ROX), and at the 3'end with one of Black Hole Quenchers (BHQ). BHQ molecules act as effective fluorescence quenchers in the entire visible and infrared spectrum, releasing energy mostly in the form of heat and not light. Probes based on the use of BHQs give a very low background and facilitate a very sensitive detection. Maximum of absorbance for BHQ-1 corresponds to 534 nm, and it quenches most efficiently in the range of 480-580 nm; while BHQ-2 has its maximum of absorbance at 579 nm,this makes possible fluorescence quenching between 550 and 650 nm.

  • Dual labelled fluorogenic probes are labelled at their 5'end with a fluorophore (FAM, TET, JOE, HEX, Fluorescein or Rhodamine), and at 3'end with a quencher (TAMRA, Dabcyl). Taqman probes (linear) are designed to quantify PCR products, detect mutations and discriminate alleles. Molecular Beacons probes (forming internal hairpins), more specific than linear probes, are used in in situ hybridisation, quantification of hybridising sequences and in allele discrimination.

Prices (PLN netto), minimum yield and offered scale of synthesis of FRET oligonucleotides and Dual labeled Fluorogenic Probes:

LPlabelling : Reporter (nm)Quencher
Abs (nm)
cena * / skala
5'3'AbsEms 5 nmol10 nmol20 nmol30 nmol50 nmol
1LC CYAN 500 TAMRA (Q) 450 500 544 9891173151822082875
2LC CYAN 500 BHQ-1 450 500 535 9891173151822082875
3LC CYAN 500 Eclipse 450 500 522 9891173151822082875
4LC CYAN 500 Dabcyl 450 500 453 9891173151822082875
5Fluo TAMRA (Q) 495 520 544 43752969011501357
6Fluo BHQ-1 495 520 535 43752969011501357
7Fluo Eclipse 495 520 522 43752969011501357
8Fluo Dabcyl 495 520 453 43752969011501357
96-FAM TAMRA (Q) 495 520 544 43752969011501357
106-FAM BHQ-1 495 520 535 43752969011501357
116-FAM Eclipse 495 520 522 43752969011501357
126-FAM Dabcyl 495 520 453 43752969011501357
13RhodamineGreen TAMRA (Q) 503 528 544 9891173151822082875
14RhodamineGreen BHQ-1 503 528 535 9891173151822082875
15RhodamineGreen Eclipse 503 528 522 9891173151822082875
16RhodamineGreen Dabcyl 503 528 453 9891173151822082875
17TET TAMRA (Q) 521 536 544 9891173151822082875
18TET BHQ-1 521 536 535 9891173151822082875
19TET Eclipse 521 536 522 9891173151822082875
20TET Dabcyl 521 536 453 9891173151822082875
21JOE TAMRA (Q) 522 548 544 713851110416102093
22JOE BHQ-1 522 548 535 713851110416102093
23JOE Eclipse 522 548 522 713851110416102093
24JOE Dabcyl 522 548 453 713851110416102093
25YakimaYellow TAMRA (Q) 530 549 544 713851110416102093
26YakimaYellow BHQ-1 530 549 535 713851110416102093
27YakimaYellow Eclipse 530 549 522 713851110416102093
28YakimaYellow Dabcyl 530 549 453 713851110416102093
29Hex TAMRA (Q) 535 556 544 713851110416102093
30Hex BHQ-1 535 556 535 713851110416102093
31Hex BHQ-2 535 556 579 713851110416102093
32Hex Eclipse 535 556 522 713851110416102093
33Hex Dabcyl 535 556 453 713851110416102093
34Cy3 BHQ-2 546 563 579 713851110416102093
35Cy3 TAMRA (Q) 546 563 544 713851110416102093
36TAMRA (R) BHQ-2 564 579 579 9891173151822082875
37TAMRA (R) Eclipse 564 579 522 9891173151822082875
38TAMRA (R) Dabcyl 564 579 453 9891173151822082875
39ROX BHQ-1 576 601 535 9891173151822082875
40ROX BHQ-2 576 601 579 9891173151822082875
41ROX Eclipse 576 601 522 9891173151822082875
42Texas Red BHQ-2 586 610 579 9891173151822082875
43Texas Red Eclipse 586 610 522 9891173151822082875
44LC Red 610 Eclipse 590 610 522 9891173151822082875
45LC Red 610 BHQ-2 590 610 579 9891173151822082875
46LC Red 640 BHQ-2 625 640 579 9891173151822082875
47LC Red 640 BHQ-3 625 640 672 9891173151822082875
48Cy5 BHQ-2 646 662 579 9801122146721852847
49Cy5 BHQ-3 646 662 672 9891173151822082875
50Cy5.5 BHQ-2 683 705 579 713851110416102093
51Cy5.5 BHQ-3 683 705 672 9891173151822082875
52IRD 700 BHQ-2 685 705 579 713851110416102093
53IRD 700 BHQ-3 685 705 672 9891173151822082875
54BHQ-1 Fluo 495 520 535 43752969011501357
55BHQ-1 6-FAM 495 520 535 43752969011501357
56BHQ-1 JOE 522 548 535 713851110416102093
57BHQ-1 Hex 535 556 535 9891173151822082875
58BHQ-1 Cy3 546 563 535 713851110416102093
59BHQ-1 ROX 576 601 535 9891173151822082875
60BHQ-2 Hex 535 556 579 9891173151822082875
61BHQ-2 ROX 576 601 579 9891173151822082875
62BHQ-2 LC Red 610 590 610 579 9891173151822082875
63BHQ-2 LC Red 640 625 640 579 9891173151822082875
64BHQ-2 Cy5 646 662 579 713851110416102093
65BHQ-2 Cy5.5 683 705 579 713851110416102093
66Eclipse Fluo 495 520 522 43752969011501357
67Eclipse 6-FAM 495 520 522 43752969011501357
68Eclipse JOE 522 548 522 713851110416102093
69Eclipse Hex 535 556 522 9891173151822082875
70Eclipse ROX 576 601 522 9891173151822082875
71Eclipse LC Red 610 590 610 522 9891173151822082875
72Dabcyl Fluo 495 520 453 43752969011501357
73Dabcyl 6-FAM 495 520 453 713851110416102093
74Dabcyl JOE 522 548 453 9891173151822082875
* HPLC purification and MALDI ToF quality control are included.

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LightCycler Probes


The LightCycler hybridisation probe system is based on two oligonucleotide probes that hybridise, in a head-to-tail arrangement, to adjacent sequences of the target DNA. Each probe is labelled with a different fluorophore, the first one with Fluorescein at the 3'end (donor), the second one with either LC610,LC640,LC670 or LC705 at the 5'end (acceptor). Additionally, 3'OH terminus of LC probes is phosphorylated to prevent polymerase from extending the primer during PCR reaction. When the fluorophores are in close proximity, there can be a fluorescence resonance energy transfer (FRET) between them, which allows a specific and quantitative real time detection of the template amplification. To avoid sterical problems between the 3' Fluo and 5' LC labels of the probes, there should be a spacer of 1 to 5 nucleotides between the two probes.

Net prices, minimal amounts / offered scale of synthesis of LightCycler probes:

 LightCycler Probes
productprice PLN netto / nmol 1/ No. of tests2
1 / 3002 / 6003 / 10005 / 150010 / 300020 / 600030 / 10000
5´LC610/640/670-3´Fosforan81992410501575205833183864
3´LC610/64081992410501575205833183864
wew.LC610/6401050117614701995247841585838
5´LC705-3´Fosforan81992410501575205833183864
3´-Fluoresceina336366399483105013021575

1 For oligonucleotides > 33 bases price for each extra base is 20 PLN.
2 Values calculated for 3 pmol of probe per assay (range of optimal amount 2-10 pmol).
* Price includes HPLC purification and MALDI ToF quality check to provide probes of exceptional purity.
* LightCycler® is a trademark of Idaho Technology Inc. Custom synthesis of LC probes and oligonucleotides containing LCRed dyes under licence of Roche Diagnostics.
* Sold under license from Hoffmann La-Roche Ltd. All products are for research only.

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RNA oligonucleotides


Our offer includes the synthesis of both labelled and standard RNA oligos.
Possible modifications comprise such molecules as TAMRA, Biotin, Amino (available for both 5' i 3' ends), Thiol for 3' end, and Phosphate, Cy3, Cy5, HEX, FAM, TET for 5' ends.

All RNA oligonucleotides are purified by HPLC.

The prices, minimum yield and offered scales of RNA oligonucleotides synthesis:

 RNA oligonucleotides
scale (µmol)price PLN netto
for oligonucleotide < 12 ntper base >= 12 nt
0.238535
1.044040


* A higher scale of synthesis - please contact us
* Prices and modifications of RNA oligonucleotides - please contact us

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peptides


We also offer the synthesis of peptides, which may be applied to isolation of protein complexes, enzymatic assays or immunization...
We offer peptides of different degrees of purity, depending on customer's needs. Peptides may be delivered bound to a solid support like sepharose for affinity chromatography We offer C-amidated or N-acetylated peptides
If you are interested in the peptide synthesis, please enquire.

 peptides
Linear PeptidesNet prices per aa (PLN)
purity / quantity2 mg5 mg10 mg20 mg50 mg100 mg
crude8-25 aan.dn.d.457092137
purity > 70%8-25 aan.d7092114160228
purity > 95%8-25 aa92114160228320432

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